The FTC-3000, a 4th generation real time PCR system, has been developed to meet the exacting demands of today’s laboratories. The small footprint instrument is a great space saver, with an embedded computer and touch screen LCD, designed for outstanding performance. The six independent temperature zoom, multi-color detection and high resolution melting(HRM) makes the FTC-3000 system very powerful.
- Specially designed six independent temperature zooms allow for a carrying capacity of up six separate amplifications at a single PCR run.
- For mutation scans and genotyping, high thermal uniformity allows for high resolution melting
- Silver module enables faster ramping speeds and performs more runs.
-Embedded computer and touch screen LCD makes the system simple, easy to operate and a spacer saver.
- User-friendly software and USB interface for data collection, analysis and management.
Unique Temperature Control System
The accuracy and uniformity of the plate temperature is the most important factor of a thermocycler.
It has developed a unique six zoom temperature control system. The temperature of the six zooms can be set up as a same temperature, which will ensure the accuracy and improve the uniformity of the plate temperature.
The system can be divided into six independent temperature zooms, i.e. the 96 well plate is divided into a 16(wells) by 6 zooms; each zoom can be set to different temperatures and controlled throughout. It can carry up to six separate amplifications in one PCR run.
Innovation Optical system
A specially designed optical system is comprised of an excitation source with extra long life, two bounds of optical fiber and a cooled CCD camera.
The broad spectrum excitation source provides high excitation energies over a wavelength range from 380 to 780nm. This arrangement makes the instrument compatible with the development of novel chemistries or fluorophores in the future.
All sample positions are illuminated and the resulting fluorescent emissions are directed through optical fibers to a cooled CCD camera. The optical signals can be collected within one second.
Powerful and professional system
1. Embedded computer and administration system
2. User friendly software with powerful functions
1) Auto-baseline and auto-threshold for simplified data analysis
2) Absolute quantification: Relative quantification △△CT:
3) Melting curve
4) Allelic Analysis △△CT
5) PCR efficiency calculation and correction well by well
6) SYBR green correction
7) Export data into Word and Excel file format
8) Quality Management
Superior temperature uniformity
Good repeats The curves for 5000 and 10000 copy template
Melting curves
Excellent detection sensitivity
Weak signal curves The curves for starting template from 10 to 1010 copies
The curves for standard samples
Specification
| Display | FTC-3000 | FTC-3000P |
| Capacity of sample | 96x0.2ml | |
| Types of sample tubes: | 8x0.2ml plate, 96x0.2ml PCR plate and standard 0.2ml thin-wall tube semi-conductor | |
| Thermal mode | Heating and cooling | |
| Temperature Range | 4-100℃ | |
| Heating speed | 5.7℃/S(MAX) | |
| Cooling speed | 4.8℃/S(MAX) | |
| Temperature display accuracy | ±0.1℃ | |
| Uniformity of well to well | ±0.25℃ | ±0.15℃ |
| Gradient Range | 30-100℃ | |
Gradient:Temperature differential range | 1-32℃ | |
| Multiple temperature zooms | 6 | |
| Gradient | Available | |
| Gradient area | 12 | |
| Detector | -20℃ cooler CCD | |
| Light Source | Halogen lamp(useful time≥5 years) | |
| Excitation/Emission light filters | 5 | |
| Total filters | 8 | |
| Excitation wave length | 380nm-780nm | |
| Emission wave length | 380nm-780nm | |
| Fluorescent | FAM.SYBR Green/Eva Green/LC Green/Fluorescein, VIC/HEX/TET/Cy3/Cy3.5/JOE/Yellow555, ROX/Texas Red, Cy5/Cy5.5/LCD Red, Tamara | |
| Functions of software | Absolute Quantification, Relative Quantification, Multiple Quantification, Melting Curve Analysis, △△CT, Allelic Analysis, PCR Efficiency calculation and correction well by well,SYBR Green I correction, Quality Management, Export easily to Word and Excel file format,Auto-baseline and auto-threshold for simplified data anlysis, Dye calibration by software | |
| Electrical | Voltage: 100-240 VAC Frequency: 50/60Hz±1% Power: 1000W | |
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