What is a Biosafety Cabinet?
Biosafety cabinets are designed to protect the operator's own, laboratory environment, and experimental materials to prevent exposure to the above-mentioned procedures when handling infectious materials such as primary cultures, strains, and diagnostic specimens. Possible aerosols and spills are possible.
effect
Prevention of the operator and the environment exposed to the experimental process of bio-aerosol generated by the negative pressure filter exhaust cabinet, is to prevent laboratory infection of the main equipment.
working principle
The air inside the cabinet to smoke out, so that the cabinet to maintain a negative pressure state, through the vertical air to protect the staff; outside air by the high efficiency air filter filter into the safety cabinet to avoid contamination of the sample; Filtered through the filter and then discharged into the atmosphere to protect the environment.
(Aerosol: refers to suspended in the gas medium, particle size is generally 0.001μm ~ 100μm solid and liquid particles formed by the gelatinous dispersion system)
What is the classification of Biosafety Cabinets?
China in 2006 formally implemented the "People's Republic of China Pharmaceutical Industry Standard: Biosafety Cabinet" (YY0569 ~ 2005), according to the air flow and isolation barrier design structure, the Biosafety Cabinet is divided into Ⅰ, Ⅱ, Ⅲ level. Our department of AIDS screening laboratory for the level of biosafety laboratory.
With the principle:
1. Level 1 Biosafety Cabinet:
Can protect the staff and the environment without protecting the sample, the air flow principle and the laboratory fume hood is basically the same, the difference is that the exhaust port is equipped with HEPA filter, the outer exhaust flow filter and thus prevent the spread of microbial aerosol pollution. A Biosafety Cabinet itself without fan, relying on external ventilation ducts in the fan-driven air, because the cabinet can not protect the product, is currently less used.
2. Secondary Biosafety Cabinet:
Currently the most widely used cabinet type, in accordance with the "People's Republic of China Pharmaceutical Industry Standard YY0569-2005 Biosafety Cabinet" in the provisions of two Biosafety Cabinets in accordance with the inlet air flow, exhaust and circulation can be divided into four levels:
A1, A2, B1 and B2, all secondary safety cabinets are available for protection of workers, the environment and the product. The minimum or measured mean of the air pressure of the front window of the A1 safety cabinet should be at least 0.38 m / S. No internal circulating airflow, 100% of the gas through the exhaust port filter discharge.
A2 type safety cabinet front window air flow speed minimum or measured average should be at least 0.5m / s. 70% of the gas is recycled to the working zone through the HEPA filter, and 30% of the gas is filtered through the exhaust port.
Two B-type Biosafety Cabinets are connected to the exhaust system safety cabinet: connected to the safety cabinet exhaust duct fan connection emergency power supply, the purpose of power can still be kept under the safety cabinet to avoid dangerous gas leaks such as laboratory The The minimum amount of the front window airflow or the average of the measurements should be at least 0.5 m / s (100 fpm).
B1-type 70% gas is removed through the exhaust port HEPA filter, and 30% of the gas is recycled to the working area through the air supply port HEPA filter.
The Type B2 is a 100% full-row safety cabinet with no internal recirculation airflow and can provide both biological and chemical safety controls that can operate volatile chemicals and volatile nuclear emissions as an additive for microbiological experiments.
3. Three-level Biosafety Cabinet:
Designed for Class 4 laboratories, the cabinet is completely airtight and the staff is operated by a glove connected to the cabinet, commonly known as a Golve box, where the test piece passes through a two-door delivery box. Cabinet to ensure that is not contaminated, suitable for high-risk biological tests, such as SARS, Ebola virus-related experiments;
According to the mandatory industry standard of Biosafety Cabinet, "People's Republic of China Pharmaceutical Industry Standard YY0569-2005 Biosafety Cabinet" provides that only the State Food and Drug Administration issued three types of medical equipment registration certificate products can really protect the operator, the experiment Room environment and operational samples; biosafety cabinets provide triple protection for people / samples and the environment.